Translation practice at the EU institutions: focus on a concordancing tool

Translation has always played a major role within the European institutions because it provides the basis for democracy and communication among the Member States and between the EU and its citizens. The enlargements brought about changes in the internal organization of the institutions – including translation services and their workflow – to respond to the new challenge of accommodating 23 official languages. A greater need for translation support was met thanks to a growing number of shared tools and resources developed over time, such as centralised web-based applications and meta-search engines. This paper focuses on one specific tool available to translators working at the EU institutions, i.e. an internally developed multilingual concordancer. Concordancers are widely used by translators but little information is available about them in terms of tool evaluation or user behaviour. This article presents a PhD research project aimed to partly fill this gap by investigating the relationship between concordance searches (seen as manifestations of translation problems) and language combination within the EU translation services

Concordancing Software in Practice: An investigation of searches and translation problems across EU official languages

2011/2012The present work reports on an empirical study aimed at investigating translation problems across multiple language pairs. In particular, the analysis is aimed at developing a methodological approach to study concordance search logs taken as manifestations of translation problems and, in a wider perspective, information needs. As search logs are a relatively unexplored data type within translation process research, a controlled environment was needed in order to carry out this exploratory analysis without incurring in additional problems caused by an excessive amount of variables. The logs were collected at the European Commission and contain a large volume of searches from English into 20 EU languages that staff translators working for the EU translation services submitted to an internally available multilingual concordancer. The study attempts to (i) identify differences in the searches (i.e. problems) based on the language pairs; and (ii) group problems into types. Furthermore, the interactions between concordance users and the tool itself have been examined to provide a translation-oriented perspective on the domain of Human-Computer Interaction. The study draws on the literature on translation problems, Information Retrieval and Web search log analysis, moving from the assumption that in the perspective of concordance searching, translation problems are best interpreted as information needs for which the concordancer is chosen as a form of external support. The structure of a concordance search is examined in all its parts and is eventually broken down into two main components: the 'Search Strategy' component and the 'Problem Unit' component. The former was further analyzed using a mainly quantitative approach, whereas the latter was addressed from a more qualitative perspective. The analysis of the Problem Unit takes into account the length of the search strings as well as their content and linguistic form, each addressed with a different methodological approach. Based on the understanding of concordance searches as manifestations of translation problems, a user- centered classification of translation-oriented information needs is developed to account for as many "problem" scenarios as possible. According to the initial expectations, different languages should experience different problems. This assumption could not be verified: the 20 different language pairs considered in this study behaved consistently on many levels and, due to the specific research environment, no definite conclusions could be reached as regards the role of the language family criterion for problem identification. The analysis of the 'Problem Unit' component has highlighted automatized support for translating Named Entities as a possible area for further research in translation technology and the development of computer-based translation support tools. Finally, the study indicates (concordance) search logs as an additional data type to be used in experiments on the translation process and for triangulation purposes, while drawing attention on the concordancer as a type of translation aid to be further fine-tuned for the needs of professional translators. ***Il presente lavoro consiste in uno studio empirico sui problemi di traduzione che emergono quando si considerano diverse coppie di lingue e in particolare sviluppa una metodologia per analizzare i log di ricerche effettuate dai traduttori in un software di concordanza (concordancer) quali manifestazioni di problemi di traduzione che, visti in una prospettiva più ampia, si possono anche considerare dei "bisogni d'informazione" (information needs). I log di ricerca costituiscono una tipologia di dato ancora relativamente nuova e inesplorata nell'ambito delle ricerche sul processo di traduzione e pertanto è emersa la necessità di svolgere un'analisi di tipo esplorativo in un contesto controllato onde evitare le problematiche aggiuntive derivanti da un numero eccessivo di variabili. I log di ricerca sono stati raccolti presso la Commissione europea e contengono quantitativi ingenti di ricerche effettuate dai traduttori impiegati presso i servizi di traduzione dell'Unione europea in un concordancer multilingue disponibile come risorsa interna. L'analisi si propone di individuare le differenze nelle ricerche (e quindi nei problemi) a seconda della coppia di lingue selezionata e di raggruppare tali problemi in tipologie. Lo studio fornisce inoltre informazioni sulle modalità di interazione tra gli utenti e il software nell'ambito di un contesto traduttivo, contribuendo alla ricerca nel campo dell'interazione uomo-macchina (Human-Computer Interaction). Il presente studio trae spunto dalla letteratura sui problemi di traduzione, sull'estrazione d'informazioni (Information Retrieval) e sulle ricerche nel Web e si propone di considerare i problemi di traduzione associati all'impiego di uno strumento per le concordanze quali bisogni di informazione per i quali lo strumento di concordanze è stato scelto come forma di supporto esterna. Ogni singola ricerca è stata esaminata e scomposta in due elementi principali: la "strategia di ricerca" (Search Strategy) e l'"unità problematica" (Problem Unit) che vengono studiati rispettivamente usando approcci prevalentemente di tipo quantitativo e qualitativo. L'analisi dell'unità problematica prende in considerazione la lunghezza, il contenuto e la forma linguistica delle stringhe, analizzando ciascuna con una metodologia di lavoro appositamente studiata. Avendo interpretato le ricerche di concordanze quali manifestazioni di bisogni d'informazione, l'analisi prosegue con la definizione di una serie di categorie di bisogni d'informazione (o problemi) legati alla traduzione e incentrati sul singolo utente al fine di includere quanti più scenari di ricerca possibile. L'assunto iniziale in base al quale lingue diverse manifesterebbero problemi diversi non è stato verificato empiricamente in quanto le 20 coppie di lingue esaminate hanno mostrato comportamenti alquanto similari nei diversi livelli di analisi. Vista la peculiarità dei dati utilizzati e la specificità dell'Unione europea come contesto di ricerca, non è stato possibile ottenere conclusioni definitive in merito al ruolo delle famiglie linguistiche quali indicatori di problemi, rispetto ad altri criteri di classificazione. L'analisi dell'unità problematica ha evidenziato le entità denominate (Named Entities) quale possibile oggetto di futuri progetti di ricerca nell'ambito delle tecnologie della traduzione. Oltre a offrire un contributo per i futuri sviluppi nell'ambito dei supporti informatici alla traduzione, con il presente studio si è voluto altresì presentare i log delle ricerche (di concordanze) quale tipologia aggiuntiva di dati per lo studio del processo di traduzione e per la triangolazione dei risultati empirico-sperimentali, cercando anche di suggerire possibili tratti migliorativi dei software di concordanza sulla base dei bisogni di informazione riscontrati nei traduttori.XXV Ciclo198

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

BACKGROUND: Pichia pastoris is a well established yeast host for heterologous protein expression, however, the physiological and genetic information about this yeast remains scanty. The lack of a published genome sequence renders DNA arrays unavailable, thereby hampering more global investigations of P. pastoris from the beginning. Here, we examine the suitability of Saccharomyces cerevisiae DNA microarrays for heterologous hybridisation with P. pastoris cDNA. RESULTS: We could show that it is possible to obtain new and valuable information about transcriptomic regulation in P. pastoris by probing S. cerevisiae DNA microarrays. The number of positive signals was about 66 % as compared to homologous S. cerevisiae hybridisation, and both the signal intensities and gene regulations correlated with high significance between data obtained from P. pastoris and S. cerevisiae samples. The differential gene expression patterns upon shift from glycerol to methanol as carbon source were investigated in more detail. Downregulation of TCA cycle genes and a decrease of genes related to ribonucleotide and ribosome synthesis were among the major effects identified. CONCLUSIONS: We could successfully demonstrate that heterologous microarray hybridisations allow deep insights into the transcriptomic regulation processes of P. pastoris. The observed downregulation of TCA cycle and ribosomal synthesis genes correlates to a significantly lower specific growth rate during the methanol feed phase

Development and subunit composition of synaptic NMDA receptors in the amygdala: NR2B Synapses in the adult central amygdala

NMDA receptors are well known to play an important role in synaptic development and plasticity. Functional NMDA receptors are heteromultimers thought to contain two NR1 subunits and two or three NR2 subunits. In central neurons, NMDA receptors at immature glutamatergic synapses contain NR2B subunits and are largely replaced by NR2A subunits with development. At mature synapses, NMDA receptors are thought to be multimers that contain either NR1/NR2A or NR1/NR2A/NR2B subunits, whereas receptors that contain only NR1/NR2B subunits are extrasynaptic. Here, we have studied the properties of NMDA receptors at glutamatergic synapses in the lateral and central amygdala. We find that NMDA receptor-mediated synaptic currents in the central amygdala in both immature and mature synapses have slow kinetics and are substantially blocked by the NR2B-selective antagonists (1S, 2S)-1-(4-hydroxyphenyl)-2-(4-hydroxy-4-phenylpiperidino)-1-propano and ifenprodil, indicating that there is no developmental change in subunit composition. In contrast, at synapses on pyramidal neurons in the lateral amygdala, whereas NMDA EPSCs at immature synapses are slow and blocked by NR2B-selective antagonists, at mature synapses their kinetics are faster and markedly less sensitive to NR2B-selective antagonists, consistent with a change from NR2B to NR2A subunits. Using real-time PCR and Western blotting, we show that in adults the ratio of levels of NR2B to NR2A subunits is greater in the central amygdala than in the lateral amygdala. These results show that the subunit composition synaptic NMDA receptors in the lateral and central amygdala undergo distinct developmental changes

Lactate production yield from engineered yeasts is dependent from the host background, the lactate dehydrogenase source and the lactate export

BACKGROUND: Metabolic pathway manipulation for improving the properties and the productivity of microorganisms is becoming a well established concept. For the production of important metabolites, but also for a better understanding of the fundamentals of cell biology, detailed studies are required. In this work we analysed the lactate production from metabolic engineered Saccharomyces cerevisiae cells expressing a heterologous lactate dehydrogenase (LDH) gene. The LDH gene expression in a budding yeast cell introduces a novel and alternative pathway for the NAD(+ )regeneration, allowing a direct reduction of the intracellular pyruvate to lactate, leading to a simultaneous accumulation of lactate and ethanol. RESULTS: Four different S. cerevisiae strains were transformed with six different wild type and one mutagenised LDH genes, in combination or not with the over-expression of a lactate transporter. The resulting yield values (grams of lactate produced per grams of glucose consumed) varied from as low as 0,0008 to as high as 0.52 g g(-1). In this respect, and to the best of our knowledge, higher redirections of the glycolysis flux have never been obtained before without any disruption and/or limitation of the competing biochemical pathways. CONCLUSION: In the present work it is shown that the redirection of the pathway towards the lactate production can be strongly modulated by the genetic background of the host cell, by the source of the heterologous Ldh enzyme, by improving its biochemical properties as well as by modulating the export of lactate in the culture media

Two-dimensional gel proteome reference map of human small intestine

<p>Abstract</p> <p>Background</p> <p>The small intestine is an important human organ that plays a central role in many physiological functions including digestion, absorption, secretion and defense. Duodenal pathologies include, for instance, the ulcer associated to Helicobacter Pylori infection, adenoma and, in genetically predisposed individuals, celiac disease. Alterations in the bowel reduce its capability to absorb nutrients, minerals and fat-soluble vitamins. Anemia and osteopenia or osteoporosis may develop as a consequence of vitamins malabsorption. Adenoma is a benign tumor that has the potential to become cancerous. Adult celiac disease patients present an overall risk of cancer that is almost twice than that found in the general population. These disease processes are not completely known.</p> <p>To date, a two dimensional (2D) reference map of proteins expressed in human duodenal tissue is not yet available: the aim of our study was to characterize the 2D protein map, and to identify proteins of duodenal mucosa of adult individuals without duodenal illness, to create a protein database. This approach, may be useful for comparing similar protein samples in different laboratories and for the molecular characterization of intestinal pathologies without recurring to the use of surgical material.</p> <p>Results</p> <p>The enrolled population comprised five selected samples (3 males and 2 females, aged 19 to 42), taken from 20 adult subjects, on their first visit at the gastroenterology unit for a suspected celiac disease, who did not turn to be affected by any duodenal pathology after gastrointestinal and histological evaluations. Proteins extracted from the five duodenal mucosal specimens were singly separated by 2D gel electrophoresis. After image analysis of each 2D gel, 179 protein spots, representing 145 unique proteins, from 218 spots tested, were successfully identified by MALDI-TOF ms analysis. Normalized volumes, for each protein, have been reported for every gel. Proteins have been grouped according to their biological/metabolic functions.</p> <p>Conclusion</p> <p>This study represents to date the first detailed and reproducible 2D protein map of human duodenum. Spots identifications, reported in a database, will be helpful to identify the variability in protein expression levels, in isoforms expression, or in post-translational modifications associated to pathology or to a therapy.</p

Favourable effects of cardiovascular rehabilitation on funcional capacity and autonomic balance in elderly patients with heart failure

In 42 patients with chronic heart failure we evaluated left ventricular function, exercise capability and autonomic control before and 3 months after a program of cardiovascular rehabilitation. The results were analyzed separately for younger (Group 1, n=18, age 51±6 years) and older patients (Group 2, n=24, age 68±4 years), with comparable clinical characteristics and therapy. Before rehabilitation, compared to younger patients, Group 2 patients showed a lower exercise capability, a comparable left ventricular ejection fraction and similar high sympathetic activity at rest, with no response to regular breathing (= stimulation of cardiopulmonary receptors, i.e. parasympathetic challenge) and active standing (= sympathetic stimulation). After rehabilitation, in both groups a 20% improvement of exercise tolerance and aerobic performance was observed, as well as a slightly increase of left ventricular ejection fraction (about 10%), and a recovery in vagal and sympathetic responsiveness. Thus, in heart failure patients age does not hinder the favorable clinical and autonomic modulation induced by cardiovascular rehabilitation

Endomicroscopy and Cancer: A New Approach to the Visualization of Neoangiogenesis

Probe-based Confocal Laser Endomicroscopy (pCLE) is a novel imaging technique for gastrointestinal endoscopy providing in vivo microscopy at subcellular resolution. It offers the possibility to analyze neoangiogenesis and vessel density in vivo. Angiogenetic switch is essential in cancer progression. Aim of the paper was to review the use of this imaging tool to analyze colorectal and gastric cancers vascularization in vivo. The aim is to provide the possibility of combining diagnostic evidences with vascularization and molecular profile to evaluate the efficacy of an antiangiogenic treatment in association with conventional therapy. pCLE can be considered a revolutionary method for real-time assessment of changes in vascularization pattern in this tumors and it may open the possibility to address the use of anti-angiogenic therapy in order to improve the outcome of the treatment

Nanocellulose/Fullerene Hybrid Films Assembled at the Air/Water Interface as Promising Functional Materials for Photo-Electrocatalysis

Cellulose nanomaterials have been widely investigated in the last decade, unveiling attractive properties for emerging applications. The ability of sulfated cellulose nanocrystals (CNCs) to guide the supramolecular organization of amphiphilic fullerene derivatives at the air/water interface has been recently highlighted. Here, we further investigated the assembly of Langmuir hybrid films that are based on the electrostatic interaction between cationic fulleropyrrolidines deposited at the air/water interface and anionic CNCs dispersed in the subphase, assessing the influence of additional negatively charged species that are dissolved in the water phase. By means of isotherm acquisition and spectroscopic measurements, we demonstrated that a tetra-sulfonated porphyrin, which was introduced in the subphase as anionic competitor, strongly inhibited the binding of CNCs to the floating fullerene layer. Nevertheless, despite the strong inhibition by anionic molecules, the mutual interaction between fulleropyrrolidines at the interface and the CNCs led to the assembly of robust hybrid films, which could be efficiently transferred onto solid substrates. Interestingly, ITO-electrodes that were modified with five-layer hybrid films exhibited enhanced electrical capacitance and produced anodic photocurrents at 0.4 V vs Ag/AgCl, whose intensity (230 nA/cm2) proved to be four times higher than the one that was observed with the sole fullerene derivative (60 nA/cm2)